B-1 cells modulate the murine macrophage response to Leishmania major infection

Figure 6 Blockage of the cyclooxygenase pathway induces protective phenotype in the Leishmania major infection. Infected macrophages from BALB/c or XID mice macrophages were incubated in the presence or absence of L. major, 24 h after the cellular cultures were washed and B-1 cells were added. The co-culture was treated or not with aspirin (AAS) (10 mg/mL). After 24 h of incubation, the cells were washed and incubated again for 3 d. After this time, the promastigotes were counted in the culture supernatant. All cultures were performed in triplicate and bars show the mean ± SD. Statistical analysis were performed by t-test from representative results of three different experiments and bars show the mean ± SD. ^aP < 0.05, ^bP < 0.005 and ^dP < 0.0001.