B-1 cells modulate the murine macrophage response to Leishmania major infection

Figure 3 Interleukin-10 is a determinant factor for the increased parasite load in macrophages. Peritoneal macrophages from BALB/c or XID mice co-cultured with B-1 cells were cultured in the presence or absence of L. major (MOI 10:1). After 24 h, the supernatant was collected and IL-10 was measured by ELISA (A). All cultures were performed in triplicate and bars show the mean ± SD. Representative result of three different experiments. ^aP < 0.05, ^bP < 0.005 and ^dP < 0.0001. Co-cultures of B-1 cells and infected macrophages from BALB/c or XID mice were treated or not with doses of monoclonal neutralizing anti-IL-10 or control isotype. Once were infected with L. major, after 24 h, the cell cultures were washed with DMEM and incubated 3 d and then passed to Schneider medium. After 5 d in medium Schneider, promastigotes were counted in the supernatant (B). Statistical analysis were performed by t-test from representative results of three different experiments and bars show the mean ± SD. ^aP < 0.05 and ^bP < 0.005.