B-1 cells modulate the murine macrophage response to Leishmania major infection

Figure 2 The immunomodulatory effect of co-cultured infected macrophages and B-1 cells is independent of contact. Peritoneal macrophages from BALB/c (A) and XID (B) mice were plated in 24 wells vessels at 105 cells/well in complete culture medium and infected with L. major (10⁶/well). After 24 h of infection, cells were washed and B-1 cells were added at same compartment (Unsep), or separated (Sep) by a cell-impermeable culture insert. The cells were cultured in Schneider medium for 5 d at a temperature of 27 °C. After this period, the promastigotes were quantified in the supernatant of the cultures of infected phagocytes. All cultures were performed in triplicate and bars show the mean ± SD. Statistical analysis were performed by t-test from representative results of three different experiments. ^aP < 0.05 and ^bP < 0.005.