B-1 cells modulate the murine macrophage response to Leishmania major infection

Figure 1 Effect of B-1 cells co-cultured with macrophages infected with Leishmania major. B-1 cells and peritoneal macrophages from BALB/c mice (white bars) or XID mice (black bars) were cultured (105/well) and infected with metacyclic promastigotes of L. major. After 24 h, the cell culture was washed and phagocytes were cultured for another 3 d with DMEM supplemented with 10% FBS at 37 °C. After this period, cells were stained and amastigotes inside the phagocytes were counted under the light microscope (A) and set the percentage of infected cells (B). To quantify promastigotes forms in the supernatants, the cells were cultures and infected with L. major. After 24 h of infection, so were washed and cultured at a temperature of 27 °C in Schneider medium 20% FBS during 5 d. After this period, the promastigotes were quantified in the supernatant of the cultures of infected phagocytes (C). All cultures were performed in triplicate and bars show the mean ± SD. Statistical analysis were performed by t-test from representative results of three different experiments. ^aP < 0.05, ^bP < 0.005 and ^dP < 0.0001.