L-carnitine protects C2C12 cells against mitochondrial superoxide overproduction and cell death

Figure 1 Protection against menadione-induced mitochondrial depolarization with L-carnitine. C2C12 cells were either untreated (white histogram) or pretreated with 500 μmol/L of L-carnitine (dark histogram) and incubated for 24 h with desired concentration of menadione (from 0 to 12 μmol/L). Mitochondria integrity was evaluated using DiOC6(3) probe staining; fluorescent cells were counted using a cytometer. Results are presented as the mean value ± SD. Two different values (P < 0.005) are indicated by a star above the two histograms.