Epigenetic alteration to activate Bmp2-Smad signaling in Raf-induced senescence

Figure 4 Genes altered commonly in Ras-/Raf-induced senescence and in mock cells. A: Commonly upregulated genes. There were 348 genes showing > 5-fold upregulation commonly in mock cells, in either of the two RasG12V cells and in either of the two RafV600E cells, compared with MEF. Genes associated with signal/secreted proteins proteins (P = 6.7 × 10^-12) or extracellular regions (P = 3.3 × 10^-9), e.g., Igfbp3 and Cd5l, were significantly enriched in these genes. B: Commonly downregulated genes. There were 80 genes, e.g., Cdc6 and Wnt4, showing < 0.2-fold downregulation commonly in mock cells, in either of two RasG12V cells and in either of two RafV600E cells, compared with MEF. Genes related to disulfide bond were significantly enriched. MEF: Mouse embryonic fibroblast.