Arctic ground squirrel neuronal progenitor cells resist oxygen and glucose deprivation-induced death

Figure 2 Cultures of arctic ground squirrel neuronal progenitor cells do not begin to express MAP2ab, characteristic of mature neurons, until 21 d in vitro. Cultures of AGS NPC express nestin, a marker of NSC, but not GFAP, a marker for astrocytes at 7 d in vitro (DIV) (top left). By 14 DIV cultures express TUJ1 a marker of neuronal progenitor cells that are committed to a neuronal fate, and GFAP a marker of progenitor cells commitment to an astrocytic fate. Nestin is no longer expressed at 14 DIV (middle left). At 21 DIV cultures express TUJ1 and begin to express MAP2ab (bottom left). AGS NPCs at 14 DIV increase in viability and appear to proliferate when deprived of oxygen or oxygen and glucose (right). When AGS NPC were deprived of oxygen or oxygen and glucose for 48 h followed by 24 h of resource replacement, cultures showed an increase in viability (A) and an increase in the proportion of neuronal progenitors (B) despite signs of cell death (C). Horizontal lines P < 0.05; ANOVA followed by a Holm-Sidak test. Mean ± SD. Only differences relative to control are shown. AGS: Arctic ground squirrel; NPC: Neuronal progenitor cell; OGD: Oxygen glucose deprivation.