Connective tissue growth factor differentially binds to members of the cystine knot superfamily and potentiates platelet-derived growth factor-B signaling in rabbit corneal fibroblast cells

Figure 3 Connective tissue growth factor is able to enhance the binding of platelet-derived growth factor-BB to platelet-derived growth factor receptorβ. SPR assay was carried out to quantitatively measure the binding of PDGF-BB to PDGFRβ. A: Sensorgrams of SPR analyses showing the binding dissociation equilibrium constant of PDGF-BB and PDGFRβ; B: Measurement of SPR response unit showed that CTGF promoted PDGF-BB/PDGFRβ interaction. A mixture of PDGF-BB (2 nmol/L) with different concentrations of CTGF (0-263.2 nmol/L) was run over PDGFRβ sensor chips. CTGF: Connective tissue growth factor; PDGF-B: Platelet-derived growth factor-B; SPR: Surface plasma resonance; PDGFRβ: Platelet-derived growth factor receptor β.