Disruption of NAD+ binding site in glyceraldehyde 3-phosphate dehydrogenase affects its intranuclear interactions

Figure 3 Phospho-T99-glyceraldehyde 3-phosphate dehydrogenase was detected in nuclei of A549 cells after genotoxic stress. A: 2D gel analysis of the cytosolic fraction extracted from A549 cells. Only a section of the membrane containing GAPDH isoforms is shown. Membranes were developed with anti-GAPDH antibody or anti-phospho-T99-GAPDH antibody in untreated and araC-treated cells. pH gradient is indicated under the bottom image; B: The nuclear fraction from A549 cells after treatment with araC. Only a section of the membrane containing GAPDH isoforms is shown. Membranes were developed with anti-GAPDH antibody (top image) or anti-phosphoT99-GAPDH antibody (bottom image). pH gradient is indicated under the bottom image. GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; 2D: Two dimensional.