Copyright
©The Author(s) 2015.
World J Biol Chem. Nov 26, 2015; 6(4): 366-378
Published online Nov 26, 2015. doi: 10.4331/wjbc.v6.i4.366
Published online Nov 26, 2015. doi: 10.4331/wjbc.v6.i4.366
Table 2 Kinetic parameters of wild type and mutated glyceraldehyde 3-phosphate dehydrogenase variants
| GAPDH variant | Km (G3P) (μmol/L) | Km (NAD+) (μmol/L) |
| Wild type | 77 ± 25.9 | 57 ± 11.1 |
| Y94A | 87 ± 33.1 | 97.4 ± 11 |
| S98A | 100 ± 36.7 | 101 ± 7.2 |
| T99A | 112 ± 44.7 | 105 ± 17.7 |
| T99I | 106 ± 38.3 | 741 ± 257 |
- Citation: Phadke M, Krynetskaia N, Mishra A, Barrero C, Merali S, Gothe SA, Krynetskiy E. Disruption of NAD+ binding site in glyceraldehyde 3-phosphate dehydrogenase affects its intranuclear interactions. World J Biol Chem 2015; 6(4): 366-378
- URL: https://www.wjgnet.com/1949-8454/full/v6/i4/366.htm
- DOI: https://dx.doi.org/10.4331/wjbc.v6.i4.366