Characterization of two alkyl hydroperoxide reductase C homologs alkyl hydroperoxide reductase C_H1 and alkyl hydroperoxide reductase C_H2 in Bacillus subtilis

Figure 10 Effects of various oxidants on AhpC_H1 expression in B. subtilis. Exponentially grown bacterial cultures were treated with indicated concentrations of H₂O₂, t-butyl hydroperoxide (tBOOH), cumene hydroperoxide (CMOOH), paraquat (PQ), and diamide for 45 min at 30 °C. Soluble proteins (5 μγ) were separated in 14% reducing SDS-PAGE gels, transferred to nitrocellulose membrane, and reacted with the polyclonal AhpC_H1 antibody. C0 and C45min indicate untreated B. subtilis at time 0 and after 45-min growth, respectively. SM, molecular weight markers (15, 20, 25, 37, 50, 75, 100, and 150 kDa).