Characterization of two alkyl hydroperoxide reductase C homologs alkyl hydroperoxide reductase C_H1 and alkyl hydroperoxide reductase C_H2 in Bacillus subtilis

Figure 8 Trx system-supported alkyl hydroperoxide peroxidase activity of the wild-type and mutant AhpC_H1 proteins. A: Peroxidase reaction was initiated by adding 2 μmol/L of the wild-type (W) or C37S, C47S, and C166S mutated AhpC_H1 enzymes to the reaction mixture containing 50 mmol/L Hepes-NaOH, pH 7.4, 1 mmol/L EDTA, 100 nmol/L of E. coli Trx reductase, 5 μmol/L of E. coli Trx, and 0.14 mM NADPH; the activity was continuously monitored by the decrease in 340-nm absorbance at 25 °C; B: Initial peroxidase activity rate of the wild-type and mutant AhpC_H1. The data are expressed as the mean ± standard error of three independent measurements.