Characterization of two alkyl hydroperoxide reductase C homologs alkyl hydroperoxide reductase C_H1 and alkyl hydroperoxide reductase C_H2 in Bacillus subtilis

Figure 6 High-performance liquid chromatography gel-filtration chromatograms of AhpC_H1 purified from B. subtilis. AhpC_H1 was loaded onto a ZORBAX^® Pro 10/300 GF450 column and eluted with 50 mmol/L phosphate buffer, pH 7.4, containing 150 mmol/L KCl. A: AhpC_H1 elution profile (bold line, 5); protein molecular mass standards: 1, apoferritin (AF, 443 kDa); 2, bovine serum albumin (BSA, 66 kDa); 3, ovalbumin (OVA, 44 kDa); 4, carbonic anhydrase (CA, 29 kDa). Inset shows a calibration curve used to determine AhpC_H1 molecular mass; B: The profiles of TCEP-reduced AhpC_H1 (AhpC_H1/TCEP) and AhpC_H1 treated with 2 mmol/L H2O2 for 30 min at 30 °C (AhpC_H1/H2O2) were compared with that of untreated AhpC_H1. Elution volume (mL) is in parentheses. Oligomeric AhpC_H1 was eluted at 9.379 mL.