Characterization of two alkyl hydroperoxide reductase C homologs alkyl hydroperoxide reductase C_H1 and alkyl hydroperoxide reductase C_H2 in Bacillus subtilis

Figure 3 Reversed-phase high-performance liquid chromatography analysis of fluorescently labeled trypsin-digested AhpC_H1. AhpC_H1 was modified with sulfhydryl-reactive DyLight 405 maleimide, digested with TPCK-treated trypsin in the presence or absence of 2 M urea, and analyzed by reversed-phase HPLC; fluorescence was monitored at 527 nm. Fluorescent peak (a) corresponds to a peptide containing free Cys residue conjugated with the fluorescent dye. W: Wild-type AhpC_H1; MC166: C37/47S double mutant; MS: Cys37/47/166S triple mutant.