Characterization of two alkyl hydroperoxide reductase C homologs alkyl hydroperoxide reductase C_H1 and alkyl hydroperoxide reductase C_H2 in Bacillus subtilis

Table 2 Oligonucleotides used for amplifying the DNA fusion construct to knockout Bacillus Prx genes

Prx null mutants	Primer names	Sequences of paired degenerated primers (5’→3’)
ΔAhpC_H1	H1_Up F	CCTTATTTCACAGATAAGCTCCA
	Kan/H1 R’	CCTATCACCTCAAATGGTTCGCTG AATGTATATTCCTCCTAAAAATGTAT
	Kan/H1 F’	CGAGCGCCTACGAGGAATTTGTATGGAGTGCATTCAATTGGTACTTG
	H1_Down R	ACGTGCAGTGTAGATCGCTGC
ΔAhpC_H2	H2_Up F	AAGCTGCGGATTTGAGTTGTCC
	Kan/H2 R’	CCTATCACCTCAAATGGTTCGCTGATGTATCCCTCCAATTTATTGTTTG
	Kan/H2 F’	CGAGCGCCTACGAGGAATTTGTAT TTCTTTCCAAGAACGAAAAGCGG
	H2_Down R	ATCTAAAATTGAGGAAAAACATCAA
ΔB_BCP	BCP_Up F	AATGGTACTGTGATCACTTCGTTT
	Kan/BCP R’	CCTATCACCTCAAATGGTTCGCTG TACGTTACCTCCGGATGTTTTTTT
	Kan/BCP F’	CGAGCGCCTACGAGGAATTTGTAT ATCTCTATGAGCCTATGCTTACTT
	BCP_Down R	AGTAGTCGATATGTGCATGCATT
ΔB_TPx	TPx_Up F	TTTTTCGCTTTGGACATGCTAT
	Kan/TPx R’	CCTATCACCTCAAATGGTTCGCTGTATAATTCCTCCCTTTTGTATGTAT
	Kan/TPx F’	CGA GCGCCTACGAGGAATTTGTAT GCAGGGAAAAAAGCTCCAGGC
	TPx_Down R	CGTCAGCTTTGAGCTCAAACG

Prx: Peroxiredoxins.