Characterization of two alkyl hydroperoxide reductase C homologs alkyl hydroperoxide reductase C_H1 and alkyl hydroperoxide reductase C_H2 in Bacillus subtilis

doi:10.4331/wjbc.v6.i3.249

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World Journal of Biological Chemistry

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World J Biol Chem. Aug 26, 2015; 6(3): 249-264
Published online Aug 26, 2015. doi: 10.4331/wjbc.v6.i3.249

Table 1 Oligonucleotides used for amplifying the genes expressing Bacillus peroxiredoxins proteins and its Cys residue-mutated proteins

	Sequence of primer for point mutation
AhpC_H1
C37S F	AGCGTATTCTCTTTCTACCCA
C37S R	TGGGTAGAAAGAGAATACGCT
C47S F	TTCTCTTTCGTATCTCCAACTGAGCTT
C47S R	AAGCTCAGTTGGAGATACGAAAGAGAA
C166S F	CCAGGTGAAGTTTCTCCGGCTAAATGG
C166S R	CCATTTAGCCGGAGAAACTTCACCTGG
AhpC_H2
C52S F	TTCACTTTTGTTTCTCCGACAGAAATT
C52S R	AATTTCTGTCGGAGAAACAAAAGTGAA
C169S F	ACTGGCGGACTCTCTCCGGCTAACTGG
C169S R	CCAGTTAGCCGGAGAGAGTCCGCCAGT
	Sequence of primer for protein expression
AhpC_H1
Forward	CCGCATATGTCTTTAATCGGTAAAGAAGATC
Reverse	CCGGTCGACTTAGATTTTACCTACTAGATCAAGG
AhpC_H2
Forward	CCGCATATGGCAGAACGTATGGTAGGTAA
Reverse	CCGGTCGACTTAAAGTGTTTTTTGGCCTGGTTT
B_BCP
Forward	CCGCATATGACTATAGAAATCGGACAAAAAC
Reverse	CCGGTCGACTTACTTTTCAGACATTTTGAGG
B_TPx
Forward	CCGCATATGGCTGAAATTACATTCAAAGGC
Reverse	CCGGTCGACTTACTTTCTAATGCAGCAGC

Citation: Cha MK, Bae YJ, Kim KJ, Park BJ, Kim IH. Characterization of two alkyl hydroperoxide reductase C homologs alkyl hydroperoxide reductase C_H1 and alkyl hydroperoxide reductase C_H2 in Bacillus subtilis. World J Biol Chem 2015; 6(3): 249-264
URL: https://www.wjgnet.com/1949-8454/full/v6/i3/249.htm
DOI: https://dx.doi.org/10.4331/wjbc.v6.i3.249