Short- and long-term effects of silver nanoparticles on human microvascular endothelial cells

Figure 4 Silver nanoparticles are cytotoxic and genotoxic for endothelial colony-forming cells. A: Endothelial colony-forming cells (ECFC) were treated with various concentrations of silver nanoparticles (Ag NP) for 72 h. MTT assay was then performed and data are expressed as the percentage of control. Data represent the mean ± SD of at least three separate experiments. P value was calculated vs untreated cells: ^bP < 0.01, ^dP < 0.001. B: Comet assay was performed on ECFC treated with Ag NP (2.5 and 5 μg/mL) for 24 h as described.