Identification of host miRNAs that may limit human rhinovirus replication

Figure 4 Human rhinovirus-1B RNA co-immunoprecipitates with AGO2 protein in BEAS-2B cells. A: Schematic representation of the rationale behind AGO2 co-IP experiments; B: BEAS-2B cells were infected with HRV-1B at MOI 0.01. Six hours post-infection cells were collected and AGO2 co-IP was performed. Plotted values represent the mean ± SD, of three independent experiments. The formula used takes into account the abundance of the RNAs before immunoprecipitation (see materials and methods). Unpaired t test was used to calculate the P values for AGO2 vs IgG control. NS: Not significant (P > 0.05); ^aP < 0.05; ^bP < 0.01. MOI: Multiplicity of infection; HRV: Human rhinovirus.