Identification of host miRNAs that may limit human rhinovirus replication

Figure 2 Prolonged DICER knock-down enhanced human rhinovirus-1B replication (preliminary experiments). BEAS-2B cells were transfected for three rounds with either a negative control siRNA or anti-DICER siRNA. Forty-eight hours after the third transfection, cells were infected with the indicated amount of HRV-1B, expressed as MOI (multiplicity of infection). Reverse transcription-quantitative polymerase chain reaction was used to quantify (A) DICER mRNA levels before infection or (B-D) HRV-1B RNA at 0, 3 or 8 h post-infection (HPI). The plotted values represent the average of qPCR duplicates from one experiment. The 0 HPI sample at MOI 0.001 was used as calibrator for all samples.