Identification of host miRNAs that may limit human rhinovirus replication

Figure 1 Prolonged knock-down of DICER is necessary in order to effectively lower the levels of mature microRNAs. Thirty nmol/L of either anti-DICER or negative control siRNA were used. A: Global timeline of the experiment; B: DICER mRNA or expression of the indicated microRNAs (miR-30a-5p, -92, -140, 155) was quantified by reverse transcription-quantitative polymerase chain reaction. The values plotted represent the mean ± SD, of three independent experiments. For all graphs, unpaired t test was used to calculate the P values for anti-DICER vs negative control siRNA samples. ^aP < 0.05; ^bP < 0.01. NS: Not significant.