Apoptosis induced by Fas signaling does not alter hepatic hepcidin expression

Figure 6 The effect of Jo2 on apoptosis, acute phase response and hepcidin gene expression in C57BL/6J mice. C57BL/6J male mice (n = 21) were injected with 0.2 μg/g b.w. and 0.32 μg/g b.w. Jo2 or saline and sacrificed 6 h later. Cell lysates and RNA isolated from the livers were employed in caspase-3 assays (A) or to synthesize cDNA as a template for SYB green qPCR assays to determine IL-6 (B) or SAA3 (C) gene expression. Hepcidin-1 (D) and hepcidin-2 (E) mRNA expression was determined by Taqman qPCR. Gene expression in Jo-2 injected mice was expressed as fold change of that in control mice.