Apoptosis induced by Fas signaling does not alter hepatic hepcidin expression

Figure 5 Chromatin immunoprecipitation assays. The binding of Stat3 or Smad4 to the hepcidin-1 promoter in the livers of C57BL/6NCR mice, which were injected with Jo2 (0.2 μg/g b.w.) or saline (control) and sacrificed 6 h later, was determined by ChIP assays, as described in Material and Methods. Total input DNA was used as control to evaluate the amount of chromatin.