Apoptosis induced by Fas signaling does not alter hepatic hepcidin expression

Figure 4 Phosphorylation of Stat3, NF-κB (P65) and Smad 1/5 in the liver. Whole cell lysates prepared from the livers of C57BL/6NCR mice injected with Jo2 (0.2 μg/g b.w.) and sacrificed after 1 or 6 h were employed for western blotting using anti-phospho--Stat3 (P-Stat3), anti-total Stat3, anti-phospho-P65 (P65) and anti-phospho-Smad1/5 antibodies, as described in Material and Methods. Anti-Gapdh antibody was used as protein loading control.