Apoptosis induced by Fas signaling does not alter hepatic hepcidin expression

Figure 3 The effect of longer Jo2 treatment on the level of apoptosis, acute phase response and hepcidin gene expression: C57BL/6NCR male mice, which were injected with Jo2 (0. 2 μg/g b.w.) or saline (control), were sacrificed 6 h later. A-C: Cell lysates and RNA isolated from the livers were employed in caspase-3 assays (A) or to synthesize cDNA as a template for SYB green qPCR assays to determine IL-6 (B) or SAA3 (C) gene expression; D, E: Hepcidin-1 (D) and hepcidin-2 (E) mRNA expression was determined by Taqman qPCR. Gene expression in Jo2-injected mice was expressed as fold change of that in control mice.