Binding of rhodopsin and rhodopsin analogues to transducin, rhodopsin kinase and arrestin-1

Figure 6 Ability of the reconstituted rhodopsin and rhodopsin analogues to serve as substrates for rhodopsin kinase. A: Coomassie blue staining; B: Autoradiography. Arrows indicate the migration of rhodopsin (Rho) and phosphorylated Rho (Rho-P); C: Densitometry of the autoradiograms shown in B. Dark and white bars correspond to experiments performed under dark and light conditions, respectively. Mean ± SD of three independent experiments are reported. Differences with P-values below 0.05 were considered significant.1: 9-cis-Rho; 2: 11-cis-Rho; 3: 13-cis-Rho.