Binding of rhodopsin and rhodopsin analogues to transducin, rhodopsin kinase and arrestin-1

Figure 5 Activation of transducin by reconstituted rhodopsin and rhodopsin analogues. Binding of guanine nucleotides to transducin was evaluated by Millipore filtration using [8-³H] GMPpNp. Dark and white bars correspond to experiments performed under dark and light conditions, respectively. Duplicate assays of three independent experiments were carried out. Mean ± SD are reported. Differences with P-values < 0.05 were considered significant.