Functional analysis of human Na+/K+-ATPase familial or sporadic hemiplegic migraine mutations expressed in Xenopus oocytes

Figure 6 Protein expression of the constructs G855R, L994del and Y1009X. A, B: Representative Western blots for the constructs G855R, L994del (A) and Y1009X (B) compared to the RD-WT enzyme and non-injected cells. Samples of total intracellular membrane (TM, left) and plasma membrane (PM, right) fractions corresponding to the protein amount of two oocytes were loaded in each lane (the number of cell equivalents serves as internal loading standard); C: Densitometric analysis of band intensities from at least four Western blots of G855R, L994del, Y1009X and RD-WT. The program ImageJ 1.44o (Wayne Rasband, United States) was used for analysis. In each experiment, signals were normalized to the intensity of the RD-WT signal from PM samples. An “a” indicates that the data point was significantly different from the RD-WT data (^aP < 0.05 vs RD-WT, Student’s t-test). Data are means ± SD.