Functional analysis of human Na+/K+-ATPase familial or sporadic hemiplegic migraine mutations expressed in Xenopus oocytes

Figure 4 Voltage dependence of translocated transient charge. A-E: Normalized Q(V) curves from ouabain-sensitive transient currents for the RD-WT enzyme (E), and for the mutants G900R (A), E902K (B), D999H (C) and K1003E (D). Fits of a Boltzmann function to the data are superimposed. Q_min and Q_max determined by the fit were used for normalization. The Boltzmann curve of the RD-WT enzyme is shown as a dotted line for comparison. Transient current signals are shown in a box for the RD-WT enzyme in panel (E). An “a” indicates that the data point was significantly different from the RD-WT data (^aP < 0.05 vs RD-WT, Student’s t-test).