Functional analysis of human Na+/K+-ATPase familial or sporadic hemiplegic migraine mutations expressed in Xenopus oocytes

Figure 2 Voltage and [K⁺]_ex dependence of stationary currents for ATP1A2 RD-WT, G900R, K902E, D999H and K1003E. A-E: Dependence of K⁺-induced stationary currents of the RD-WT enzyme (E) and the mutants G900R (A), E902K (B), D999H (C) and K1003E (D) on the extracellular K⁺ concentration and on membrane potential. [K⁺]_ex-dependent currents were calculated as the difference between currents induced by voltage steps first in presence of different [K⁺]_ex and then at [K⁺]_ex = 0. The amplitudes at [K⁺]_ex = 10 mmol/L and 0 mV were used for normalization. Different [K⁺]_ex are indicated by symbols. The RD-WT curve at 10 mmol/L K⁺ is superimposed as dotted line for comparison. Data are means ± SE obtained from 5-15 cells of at least three batches.