High-density lipoprotein endocytosis in endothelial cells

Figure 4 High density lipoprotein uptake in non-parenchymal liver cells. Fluorescently labeled high density lipoprotein (HDL)-Alexa 568 was intravenously injected into C57BL/6 mice. After 60 min, tissues were fixed by transcardial perfusion. Liver sections were stained for cellular markers by immunofluorescence and analyzed by confocal microscopy. HDL is localized in endothelial cells [A and B (inset)] and to a limited amount in Kupffer cells (C), whereas stellate cells show no detectable HDL staining (D).