Effect of alcohol exposure on hepatic superoxide generation and hepcidin expression

Figure 8 Detection of apoptosis in Sod2^+/- mice with alcohol exposure. Liver protein lysates, isolated from mitochondria (A) or cytosol (B) of littermate control (LMC) and Sod2^+/- mice fed with 10% ethanol (EtOH) or plain water (H₂O), were resolved by SDS-Polyacrylamide gel electrophoresis and subjected to western blotting by using an anti-cytochrome c antibody. Anti-COX4 (A) and gapdh (B) antibodies were used as loading controls; C: Whole cell lysate (WCL) proteins isolated from the livers of LMC and Sod2^+/- mice fed with 10% ethanol or plain water were employed to measure caspase-3 activity, as described in Methods. WCL isolated from the livers of C57BL/6 strain wild-type male mice 6 h after the injection (ip) of anti-mouse Fas agonist antibody, Jo2 (0.32 μg/g. b.w., Millipore) were used as internal control for caspase-3 assays.