Effect of alcohol exposure on hepatic superoxide generation and hepcidin expression

Figure 7 CuZn superoxide dismutase (SOD1) expression. A: Whole cell lysate proteins, isolated from the hepatocytes of Sod2^+/- and littermate control mice livers treated with 10% ethanol or plain water (H₂O) for 1 wk, were resolved by SDS-Polyacrylamide gel electrophoresis and subjected to western blotting by using an antibody against mouse SOD1. An anti-gapdh antibody was used as the loading control; B: Autoradiographs were scanned, and SOD1 expression was quantified by normalizing to gapdh expression. Normalized protein expression in water-fed Sod2^+/- and alcohol-fed Sod2^+/- and control mice was expressed as fold expression of that in water-fed control mice. LMC: Littermate control mice.