Effect of alcohol exposure on hepatic superoxide generation and hepcidin expression

Figure 6 Manganese superoxide dismutase (SOD2) expression. A: Whole cell lysate proteins, isolated from the hepatocytes of Sod2^+/- and littermate control mice (LMC) mice livers treated with 10% ethanol or plain water (H₂O) for 1 wk, were resolved by SDS-Polyacrylamide gel electrophoresis and subjected to western blotting by using an antibody against mouse SOD2. An anti-gapdh antibody was used as control to confirm equal protein loading; B: Autoradiographs were scanned, and SOD2 expression was quantified by normalizing to gapdh protein expression. Normalized protein expression in water-fed Sod2^+/- and alcohol-fed Sod2^+/- or littermate control mice was expressed as fold expression of that in water-fed littermate control mice. The letter a indicates statistical significance (^aP < 0.05).