Effect of alcohol exposure on hepatic superoxide generation and hepcidin expression

Figure 3 Alcohol and hepcidin mRNA expression. cDNA, synthesized from RNA isolated from the livers of Sod2^+/- and littermate control mice (LMC) mice fed with 10% ethanol or plain water (H₂O) for one week, was used as a template for real-time quantitative polymerase chain reaction. Hepcidin mRNA expression in alcohol-treated LMC and Sod2^+/- mice was expressed as fold expression of that in water-treated LMC mice. The letter a indicates statistical significance (^aP < 0.05).