Refractoriness of interferon-beta signaling through NOD1 pathway in mouse respiratory epithelial cells using the anticancer xanthone compound

Figure 6 High-mobility group box 1/2/3 pathway did not involved in 5,6-dimethylxanthenone-4-acetic acid-induced interferon-β expression. A-C: C10 cells were transfected with control small interfering RNA (siRNA) or high-mobility group box 1/2/3 (HMGB1/2/3) siRNA, the relative of each HMGB mRNA level was measured by real time reverse transcription-polymerase chain reaction (RT-PCR), respectively, HMGB1, HMGB2 and HMGB3; D: C10 cells were transfected with control siRNA or HMGB1/2/3 siRNA, then cells were further incubated with or without DMXAA or Poly I-C. The relative interferon (interferon) mRNA levels were measured by real time RT-PCR (^bP < 0.01 vs HMGB1/2/3 siRNA; ^dP < 0.01 vs HMGB1/2/3 siRNA in Poly I-C group).