Refractoriness of interferon-beta signaling through NOD1 pathway in mouse respiratory epithelial cells using the anticancer xanthone compound

Figure 4 5,6-dimethylxanthenone-4-acetic acid induces refractoriness of interferon-β signaling in vivo. Mice were rechallenged with 5,6-dimethylxanthenone-4-acetic acid (DMXAA) 3 h after pretreatment with DMXAA for 7 d, relative interferon (IFN)-β, 2’,5’-oligoadenylate synthetase 1 (OAS1) and myxovirus resistance 1 (Mx1), inducible protein-10 (IP10), and nucleotide oligomerization domain 1 (NOD1) mRNA levels of nasal epithelia were measured by real time reverse transcription-polymerase chain reaction. The results presented as mean ± SE (^bP < 0.01 vs DMXAA group, 5 mice each group).