Refractoriness of interferon-beta signaling through NOD1 pathway in mouse respiratory epithelial cells using the anticancer xanthone compound

Figure 3 5,6-dimethylxanthenone-4-acetic acid pretreatment of C10 mouse bronchial epithelial cells induces refractoriness to secondary 5,6-dimethylxanthenone-4-acetic acid administration. Cells were rechallenged with same concentration of 5,6-dimethylxanthenone-4-acetic acid (DMXAA) 3 h after pretreatment with DMXAA 100 μg/mL for 72 h. The total RNA was isolated and the relative inducible protein-10 (IP10) (A, B), Rantes (C,D), interferon (IFN)-β (E, F) and nucleotide oligomerization domain 1 (NOD1) (G,H) mRNA levels were measured by real time reverse transcription-polymerase chain reaction. The results presented as mean ± SE (^bP < 0.01 vs DMXAA groups).