Refractoriness of interferon-beta signaling through NOD1 pathway in mouse respiratory epithelial cells using the anticancer xanthone compound

Figure 2 5,6-dimethylxanthenone-4-acetic acid activates interferon-β-mediated antiviral genes and other chemokine/cytokine levels in mouse bone marrow derived dendritic cells. Cells were incubated with or without 5,6-dimethylxanthenone-4-acetic acid (DMXAA) 100 μg/mL, lipopolysaccharide (LPS) 100 ng/mL and CpG DNA 0.5 μmol/L for 6 h, respectively. The total RNA was isolated and the relative interferon (IFN)-β, 2’,5’-oligoadenylate synthetase 1 (OAS1) and myxovirus resistance 1 (Mx1), inducible protein-10 (IP10), macrophage chemotactic protein (MCP1) mRNA levels were measured by real time reverse transcription-polymerase chain reaction. The results presented as mean ± SE (^bP < 0.01 vs DMXAA, LPS and CpG DNA, respectively).