Lipopolysaccharide triggers nuclear import of Lpcat1 to regulate inducible gene expression in lung epithelia

Figure 2 Haemophilus influenzae and Escherichia coli trigger Lpcat1 nuclear translocation. MLE cells were exposed to LPS-containing bacteria at a concentration of 10⁹/mL for 4 h. The infected cells were then washed and cytosolic and nuclear protein fractionations were prepared. Immunoblotting analysis was performed using antibodies as indicated. The results represent three independent experiments. “C” donates to cytosolic fraction and “N” represents the nuclear fraction. Lamin A/C is used as a nuclear fraction protein marker and β-actin is the cytosolic fraction protein marker. LPS: Lipopolysaccharide.