Lipopolysaccharide triggers nuclear import of Lpcat1 to regulate inducible gene expression in lung epithelia

Figure 1 Lpcat1 shifts into the nucleus in lipopolysaccharide treated lung epithelia. MLE cells were treated with 10 μg/mL of LPS for 2 h. The harvested cells were processed for cytosolic and nuclear protein fractionation and the fractions were subjected to immunoblotting with different antibodies as indicated. “C” donates to cytosolic fraction and “N” represents the nuclear fraction. Lamin A/C is used as a nuclear fraction protein marker and β-actin is the cytosolic fraction protein marker. The results represent three independent experiments. LPS: Lipopolysaccharide.