Lipopolysaccharide triggers nuclear import of Lpcat1 to regulate inducible gene expression in lung epithelia

doi:10.4331/wjbc.v3.i7.159

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World Journal of Biological Chemistry

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1949-8454

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Biol Chem. Jul 26, 2012; 3(7): 159-166
Published online Jul 26, 2012. doi: 10.4331/wjbc.v3.i7.159

Table 1 Primers used in quantitative polymerase chain reaction

Gene symbol	Forward primer	Reverse primer
L19	AGAGCTTGGAGTGCACAGGT	GGGACAGTCCAAACTGGGTA
TGF-b1	GACTCTCCACCTGCAAGACC	GACTGGCGAGCCTTAGTTTG
IL-13Ra1	CACAGAAGTTCAGCCACCTG	AATTTGGACTGGCTCCTTCA
ILRN	GTTGGAAGGCAGTGGAAGAC	GCATCTTGCAGGGTCTTTTC
IL4	CCAAGGTGCTTCGCATATTT	ATCGAAAAGCCCGAAAGAGT
SPHK1	GCTGTGAGGCTGGTGTTATG	ATATGCTTGCCCTTCTGCAT
EDG7	ATTGCCTCTGCAACATCTCG	ATGAAGAAGGCCAGGAGGTT
IL-13Ra2	TTGGTCTGCTCTTGGAAACC	CAGCACACTGTAAGGCATGA
Autotaxin	GAAACAGCACCTTCCCAAAC	AAGGTTTCCTTGCAACATGC
LPA4	ACTGCGTTCCTCACCAACAT	CGATCGGAAGGGATAGACAA
sST2	CATGGCATGATAAGGCACAC	GATGCAGTGCACAGCTGATT
LPCAT1	CGACTGAGCGCCCTGCAGAA	AAGGGCCAGGCCAGCAGCAT
ST2L	GGAACGATGGCAAGCTCTAC	GGCAGAGTGTGGTGAACAAA
EDG1	CTGTTAGATGTGGGCTGCAA	ATGATGGGGTTGGTACCTGA
MMP9	CTTCGAGGGACGCTCCTATT	CAAATTTGCCGTCCTTATCG
IL-1β	CAGGCAGGCAGTATCACTCA	AGGTGCTCATGTCCTCATCC
IL33	CCTTCTCGCTGATTTCCAAG	GCTGAACAGAACGTGTGCAT
IL-13	CAGCAGCTTGAGCACATTTC	ATAGGCAGCAAACCATGTCC
EDG8	ACACCAAATGCCCAGCTTAC	TGGAGCACTGTGCAAAAGTC
EDG2	TCAACCTGGTGACCTTTGTG	GGTCCAGAACTATGCCGAGA
IL4R	TCACAGAGCAGCCTTCACAC	TTGTCTGCAAGGACAAGTGG
LPA5	GCTCCAGTGCCCTGACTATC	CAGAGCGTTGAGAGGGAGAC
CCTa	GGAAACTCCGGGTAGTGGGTGC	TTCCCTAGCTGTCACTGGCCCA
COX2	CCCCCACAGTCAAAGACACT	GGCACCAGACCAAAGACTTC
CTTN	AGCAGAGATGGGGTGCTAAA	TTGAGCGTCTGGTGTTCTTG
SGPP2	CATCTCCTTCACCCTCCTCA	CCAGCGTAGAGAACACCACA
SGPL1	AACTCTGCCTGCTCAGGGTA	CTCCTGAGGCTTTCCCTTCT
IL-6	CCGGAGAGGAGACTTCACAG	TCCACGATTTCCCAGAGAAC
MET	CAGCCATCCCAATGTTCTCT	AATTTCGCAGATCTCCATGC
EDG3	TGCCTTATGAACCCTGGAAG	AAGAGCAAATGCCATCAGGT
EDG6	CTCCAAGGGCTATGTGCTCT	ATTGGCTCGGACCACTCTAA
CADHERIN	GCACTCTTCTCCTGGTCCTG	TATGAGGCTGTGGGTTCCTC
SGPP1	GAGCAACTTGCCCGCTCTACT	AAGGGGTCGAGATTCCAGAT
SPHK2	ACTGCTCGCTTCTTCTCTGC	GCCACTGACAGGAAGGAAAA

The sequences of all the primers are from 5’- to -3’.

Citation: Ellis B, Kaercher L, Snavely C, Zhao Y, Zou C. Lipopolysaccharide triggers nuclear import of Lpcat1 to regulate inducible gene expression in lung epithelia. World J Biol Chem 2012; 3(7): 159-166
URL: https://www.wjgnet.com/1949-8454/full/v3/i7/159.htm
DOI: https://dx.doi.org/10.4331/wjbc.v3.i7.159