Review
Copyright ©2012 Baishideng Publishing Group Co.
World J Biol Chem. Jul 26, 2012; 3(7): 127-158
Published online Jul 26, 2012. doi: 10.4331/wjbc.v3.i7.127
Table 3 Pattern of expression of transient receptor potential vanilloid channels in vascular endothelial cells as detected by reverse transcription/polymerase chain reaction, immunostaining for cultured cells, immunohistochemistry, Western blotting, and in situ hybridization
TRPV1TRPV2TRPV3TRPV4TRPV5TRPV6
PCa/PNa and conductance (pS)[202]10, 35-801-3, NM12, 1726, 90> 100, 75> 100, 40-70
Human coronary artery ECs[300](+RT-PCR, WB, IHC)
Human pulmonary artery ECs[415]+(RT-PCR)+(RT-PCR)+(RT-PCR)
Human pulmonary microvascular ECs+(RT-PCR)
Human cerebral microvascular ECs[272]+(RT-PCR, IC)
Human cerebral arterioles ECs[305]+(RT-PCR, IHC)
Human dermal microvascular ECs[319]+(RT-PCR, WB)
Breast cancer derived microvascular ECs[319]+(RT-PCR, WB)
Human umbilical vein ECs[227,277,296,301]+(RT-PCR)+(WB, IHC)
Mouse aortic ECs[280,285,299,306]+(WB)+(RT-PCR, WB, NM, IHC)
Mouse pulmonary artery ECs[313]+(WB, IHC)
Mouse mesenteric artery ECs[27,280,302]+(RT-PCR, WB, IC)+(RT-PCR, IC)
Mouse cerebral microvascular ECs+(RT-PCR)+(RT-PCR, WB)+(RT-PCR)
Mouse dermal microvascular ECs[307]+(RT-PCR, WB)
Mouse carotid artery ECs[290,296]+(IHC)
Rat mesenteric artery ECs[226,275,302]+(WB)+(WB, IC, IHC)
Rat femoral artery ECs[312]+(RT-PCR, IC)
Rat pulmonary artery ECs[303]+(WB, IHC)
Rat renal artery ECs[303]+(IHC)
Rat cardiac microvascular ECs[303,316]+(IHC)
Rat cerebral artery ECs[287,297,311]+(RT-PCR)+(RT-PCR, IC)+(RT-PCR, IC)
Rat carotid artery ECs[291]+(RT-PCR)
Porcine aortic ECs[312,317]+(RT-PCR, IC, WB)
Porcine coronary artery ECs[273]+(IHC)
Bovine aortic ECs[271]+(WB)
Bovine adrenal capillary ECs[292]+(RT-PCR, WB)
Zebrafish ventricular ECs+(ISH)
RT-PCR: Reverse transcription/polymerase chain reaction; WB:Western blotting; IC: Immunostaining for cultured cells; IHC: Immunohistochemistry; ISH: In situ hybridization; NM: Not measured; ECs: Endothelial cells.