Update on vascular endothelial Ca2+ signalling: A tale of ion channels, pumps and transporters

Table 2 Pattern of expression of transient receptor potential canonical channels in vascular endothelial cells as detected by reverse transcription/polymerase chain reaction, immunostaining for cultured cells, immunohistochemistry, Western blotting, northern blots, and in situ hybridization

	TRPC1	TRPC2	TRPC3	TRPC4	TRPC5	TRPC6	TRPC7
PCa/PNa and conductance (pS)[202]	Non-selective, 16	2.7, 42	1.6, 60-66	1.1, 30-42	9, 47-66	5, 28-37	0.5-5.4, 25-50
Human pulmonary artery ECs[210,211,219,222,249,261,415,416]	+(RT-PCR, NB, WB, IC)		+/-(RT-PCR)	+(RT-PCR, WB, IC)	-(RT-PCR)	+/-(RT-PCR) +(WB)
Human coronary artery ECs[234,237]	+(RT-PCR, WB, ISH, IHC)		+(RT-PCR, WB, ISH, IHC)	+(RT-PCR, WB, ISH, IHC)	+(RT-PCR, WB, ISH, IHC)	+(RT-PCR, WB, ISH, IHC)	+(RT-PCR, WB, ISH)
Human cerebral artery ECs	+(RT-PCR, ISH, IHC)		+(RT-PCR, ISH, IHC)	+(RT-PCR, ISH, IHC)	+(RT-PCR, ISH, IHC)	+(RT-PCR, ISH, IHC)	+(RT-PCR, ISH)
Human intact mesenteric artery[127]	+(RT-PCR)		+(RT-PCR)	-(RT-PCR)	-(RT-PCR)
Human glomerular ECs						+(WB, IC)
Human preglomerular ECs[244,245]			+(RT-PCR, WB, IHC)			+(IHC)
Human dermal microvascular ECs[18,212,221,222,264]	+(RT-PCR, WB)	-(RT-PCR)	+(RT-PCR)	+(RT-PCR, WB)	-(RT-PCR)	+/-(RT-PCR) +(WB)	+/-(RT-PCR)
HUVECs[127,205,212,215,218,220-223,227,231]	+(RT-PCR, WB)	-(RT-PCR)	+/-(RT-PCR)	+/-(RT-PCR)	+/-(RT-PCR)	+/-(RT-PCR)	+(RT-PCR)
						+(WB)
EA.hy926 cells[127,243,269]	+(RT-PCR)		+/-(RT-PCR) +(WB)	+/-(RT-PCR)	+(RT-PCR, WB)	+/-(RT-PCR)
Mouse aortic ECs[216,228,248,258]	+(RT-PCR, WB, IC)		+(WB)	+(RT-PCR, WB)	+(WB)	+(WB)
Mouse pulmonary artery ECs[218,251,259,262]	+(RT-PCR, WB)	-(RT-PCR)	+/-(RT-PCR)	+(RT-PCR, WB)	-(RT-PCR)	+(RT-PCR, WB)	-(RT-PCR)
Mouse brain capillary ECs	+(RT-PCR)	+(RT-PCR)	+(RT-PCR)	+(RT-PCR)	+(RT-PCR)	+(RT-PCR)	+(RT-PCR)
Bovine pulmonary artery ECs[232]	+(RT-PCR)		-(RT-PCR)	+(RT-PCR)
Bovine aortic ECs[207-209,248,267,268]	+(RT-PCR, WB, IC)		+/-(RT-PCR) +(WB)	+(RT-PCR, WB)	+(RT-PCR, WB)	+(RT-PCR, WB)
Bovine brain capillary ECs	+(RT-PCR)	-(RT-PCR)	+(RT-PCR)	-(RT-PCR)	+(RT-PCR)	-(RT-PCR)	-(RT-PCR)
Bovine adrenal capillary ECs[292]
Rat aortic ECs[233,239]	-(WB)		+(WB)	-(WB)	+(WB)	+(WB)
Rat pulmonary artery ECs[260]	+(RT-PCR)	+(RT-PCR)	+/-(RT-PCR)	+(WB, IC)		-(RT-PCR)	-(RT-PCR)
				-(RT-PCR)
Rat coronary arterioles ECs	+(RT-PCR, IHC)
Rat cerebral artery ECs[311]	+(RT-PCR)
Rat mesenteric artery ECs[227,312]	+(RT-PCR, WB, IHC)		+(WB, IHC)	+(WB, IHC)	+(WB, IHC)
Rat descending vasa recta ECs[254]				+(RT-PCR, WB, IC)	-(RT-PCR)
Rat splenic sinus ECs[35]	+(IHC)
Porcine aortic ECs[241,242]			+(RT-PCR, WB)	+(WB)
Porcine coronary artery ECs[235]			+(RT-PCR, WB)
Zebrafish aortic ECs[225]	+(IHC, ISH)					+(ISH)
Ovine uterine artery ECs[414]	+(WB)		+(WB)	+(WB)	+(WB)	+(WB)

RT-PCR: Reverse transcription/polymerase chain reaction; WB: Western blotting; IC: Immunostaining for cultured cells; IHC: Immunohistochemistry; ISH: In situ hybridization; NB: Northern blotting; ECs: Endothelial cells; HUVECs: Human umbilical vein ECs.