Septin 8 is an interaction partner and in vitro substrate of MK5

Figure 4 Mitogen-activated protein kinase-activated protein kinase 5 colocalizes with SEPT8. A: HeLa cells were transfected separately with an expression plasmid for GFP-mitogen-activated protein kinase-activated protein kinase 5 (MK5) (left panel) or AsRed-SEPT8 (right panel); B: HeLa cells cotransfected with both expression plasmids. The subcellular localization of RFP-tagged SEPT8 (left panel), GFP-tagged MK5 (middle panel), or both proteins (merged; right panel) is shown; C: PC12 cells cotransfected with expression vectors for AsRed-SEPT8 (left panel) and GFP-MK5 (middle panel). Colocalization is shown in the right panel; D: Enlargement of the areas marked by arrows in figures (C) shows clear colocalization of MK5 and SEPT8. After 24 h, cells were fixed and MK5 (green channel) and SEPT8 (red channel) were visualized directly.