Biochemical analysis of the interactions of IQGAP1 C-terminal domain with CDC42

doi:10.4331/wjbc.v3.i3.53

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World Journal of Biological Chemistry

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1949-8454

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Biol Chem. Mar 26, 2012; 3(3): 53-60
Published online Mar 26, 2012. doi: 10.4331/wjbc.v3.i3.53

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Figure 3 Interaction between IQGAP1 and CDC42. An interaction between IQGAP1(DR6) and CDC42 can be detected by EDC crosslinking in the presence of GTP. In the absence of EDC (lanes headed -EDC), a mixture of IQGAP(DR6) and CDC42 (lane C) behaves the same on SDS-PAGE as IQGAP1(DR6) and nucleotide-depleted CDC42 (lane ND) and as IQGAP1(DR6) and GTP-loaded CDC42 (lane GTP). In the presence of the crosslinker (lanes headed +EDC), crosslinking was not observed between IQGAP(DR6) and CDC42 (C) or IQGAP(DR6) and nucleotide-depleted CDC42 (ND). However an additional band, corresponding to approximately the combined molecular masses of IQGAP(DR6) and CDC42 is seen with GTP-loaded CDC42 (GTP).

Citation: Elliott SF, Allen G, Timson DJ. Biochemical analysis of the interactions of IQGAP1 C-terminal domain with CDC42. World J Biol Chem 2012; 3(3): 53-60
URL: https://www.wjgnet.com/1949-8454/full/v3/i3/53.htm
DOI: https://dx.doi.org/10.4331/wjbc.v3.i3.53