Biochemical analysis of the interactions of IQGAP1 C-terminal domain with CDC42

Figure 2 Proteins used in this study. The recombinant expression and purification of (A) IQGAP1(DR6) and (B) CDC42 monitored by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). In both (A) and (B), U, extract from cells prior to induction; I, extract from cells 2-3 h after induction; S, soluble material remaining after sonication; F, material which flowed through the column without binding; W, material removed in the washing steps; E, elutions; M, molecular mass markers (with their masses shown to the side of the gel in kDa). In the case of CDC42, F and W were combined into a single sample.