Emanuel Strehler’s work on calcium pumps and calcium signaling

Figure 2 Scheme of the plasma membrane calcium pump and of the alternative splice options generated from the four plasma membrane calcium pump genes. Top: Scheme of the plasma membrane calcium pump (PMCA). The transmembrane regions are numbered 1-10, and arrows indicate the sites affected by alternative splicing. Splicing at site C affects the calmodulin-binding domain (CBD) and can result in different C tails due to a shift in reading frame (indicated as separate lines). Only splice variants of the “b-type” carry a PDZ domain recognition sequence (PDZ) at the C terminus. N: N terminus; C: C terminus; Bottom: Exon structure of the regions involved in alternative splicing in the four human PMCA genes. Constitutively spliced exons are shown as gray boxes. The sizes of alternatively spliced exons (colored) are given in nucleotides, the alternative splicing options are indicated by connecting lines, and the resultant splice products are labeled by their lower case symbol. Combinatorial use of the splice options at sites A and C yields over 30 possible PMCA splice variants.