Recent perspectives into biochemistry of decavanadate

Figure 5 Exchange of bound ε-ATP of G-actin with ATP. Actin monomers (5 μmol/L) were incubated for 20 min with 0-200 μmol/L decavanadate, in 2 mmol/L Tris-HCl (pH 7.5), 0.2 mmol/L CaCl₂. The nucleotide exchange was monitored by the fluorescence decrease (λex = 350 nm; λem = 410 nm), as ε-ATP was replaced by ATP. Data are plotted as mean ± SD. The results shown are the average of triplicate experiments.