Gelatin degradation assay reveals MMP-9 inhibitors and function of O-glycosylated domain

Figure 4 Dose-response curves of the inhibitory activities of SB-3CT, BB-94 and EGCG. With GraphPad prism software, the IC₅₀ of SB-3CT and BB-94 was predicted to be in the nmol/L range and the IC₅₀ of EGCG in the μmol/L range. The data points correspond to inhibitor concentrations of: 1.25, 2.5, 5, 10 and 20 μmol/L, respectively. The horizontal line shows the enzyme velocity in the absence of inhibitor.