Gelatin degradation assay reveals MMP-9 inhibitors and function of O-glycosylated domain

Table 2 Michaelis-Menten parameters for different enzyme variants

	MMP-9 FL	MMP-9 ∆Hem	MMP-9 ∆OGHem	MMP-9 ∆OG	MMP-9 MutEC
Vmax (nmol/L per minute)	3.643	2.686	2.314	1.117	-
kcat/KM (nmol/L per minute)	0.097	0.086	0.088	0.058	-
Goodness of fit (R2)	0.9977	0.9900	0.9861	0.9470	0.7096
Difference from MMP-9 FL	-	P = 0.0207	P = 0.0049	P = 0.0020	P = 0.0010

The corresponding Michaelis-Menten curves are shown in Figure 3. The P-values were calculated with a Wilcoxon signed rank test. The V_max and KM values could not be determined for the matrix metalloproteinase (MMP)-9 MutEC.